成纖維細胞是來源于中胚層的間充質細胞。乳腺的細胞外基質大部分為乳腺成纖維細胞（MF）所分泌。更重要的是這些細胞外基質通過影響基因表達，調節乳腺上皮細胞的增殖和分化。另外，有研究表明乳腺成纖維細胞能招募巨噬細胞的免疫監視，這與細胞外基質產生、新生血管生成等共同作用，形成乳腺微環境，促進腫瘤細胞的發展。這些結果表明，MF能夠刺激乳腺癌的發展和侵襲。
Sciencell研究實驗室的MF是從人乳腺分離得到的，原代凍存，冰凍運輸。每管細胞密度超過5×10^5/ml。細胞特性經纖維粘連蛋白CD90、vimentin免疫熒光鑒定。本細胞經檢測不含HIV-1、HBV、HCV、支原體、細菌、酵母菌和真菌。如采用ScienCell 實驗室特制的培養基，可保證此細胞15倍增增殖。
推薦培養基：成纖維細胞培養基 (FM, Cat. #2301)
Question:
Dear Sir, I would like to ask few questions. 1. What is the maximum passage number I can subculture the cells to, such that there is no significant genotypic or phenotypic change compared to cells at passage 0 as I received initially? 2. Is there a possibility to mix the cells from 3 donors into one vial with total cells of let say about 1 million cells/vial? Thank you very much for your time and attention. Would like to hear from you. Best regards, Aurino
Answers:
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As stated in the product sheet, Human Mammary Fibroblasts are guaranteed to expand for 15 population doublings under the conditions provided. Please note that population doubling is not the same as passage number – for help, please see our Frequently Asked Questions section under Technical Resources. We do not have quantitative data measuring phenotypic or genetic changes over passage number. Unfortunately, each lot of cells is derived from one donor, so we cannot provide one vial of cells pooled from multiple donors. However, customers can request vials from different donors when purchasing more than one vial.
