星形膠質(zhì)細(xì)胞培養(yǎng)基是專門(mén)為正常人類星形膠質(zhì)細(xì)胞體外培養(yǎng)設(shè)計(jì)的*適于其生長(zhǎng)的培養(yǎng)基。經(jīng)滅菌的液體培養(yǎng)基,包含必需和非必需氨基酸、維生素、有機(jī)和無(wú)機(jī)化合物、激素、生長(zhǎng)因子、微量礦物質(zhì)和低濃度胎牛血清(2%)。該培養(yǎng)基緩沖體系為重碳酸鹽,在含5%CO2的細(xì)胞培養(yǎng)箱中平衡后pH值為7.4。該培養(yǎng)基的配方能夠選擇性的促進(jìn)正常人類微血管內(nèi)皮細(xì)胞體外培養(yǎng)中的增殖和生長(zhǎng),并為其達(dá)到*理想營(yíng)養(yǎng)平衡狀態(tài)提供數(shù)量上和質(zhì)量上的保證。
星形膠質(zhì)細(xì)胞培養(yǎng)基包含500 ml基礎(chǔ)培養(yǎng)基,10ml胎牛血清(FBS,目錄編號(hào)0010),5ml星形膠質(zhì)細(xì)胞生長(zhǎng)添加物(AGS,目錄編號(hào)1852),和5 ml青霉素/鏈霉素溶液(P/S,目錄編號(hào)0503)
貨號(hào)
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1801
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產(chǎn)地
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美國(guó)
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縮寫(xiě)
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AM
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規(guī)格
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500ml
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用途
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科研
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儲(chǔ)存
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4度,-20度
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運(yùn)輸
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膠冰
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關(guān)鍵字
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星形膠質(zhì)細(xì)胞培養(yǎng)基 AM 星形膠質(zhì)細(xì)胞培養(yǎng)基 AM 星形膠質(zhì)細(xì)胞培養(yǎng)基 AM 星形膠質(zhì)細(xì)胞培養(yǎng)基 AM 星形膠質(zhì)細(xì)胞培養(yǎng)基 AM 星形膠質(zhì)細(xì)胞培養(yǎng)基 AM 星形膠質(zhì)細(xì)胞培養(yǎng)基 AM 星形膠質(zhì)細(xì)胞培養(yǎng)基 AM
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公司簡(jiǎn)介
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合肥星肽生物科技有限公司作為專業(yè)的ScienCell中國(guó)代理商,專業(yè)經(jīng)營(yíng)實(shí)驗(yàn)室科研用原代細(xì)胞、原代細(xì)胞專用培養(yǎng)基、原代細(xì)胞無(wú)血清培養(yǎng)基、干細(xì)胞、干細(xì)胞培養(yǎng)基、干細(xì)胞無(wú)血清培養(yǎng)基的研究和開(kāi)發(fā)。合肥星肽生物科技有限公司始終致力于為客戶提供可靠的研究材料以及方便快捷的服務(wù),對(duì)購(gòu)買(mǎi)項(xiàng)目的前期資料提供,中期合同保證,后期貨物跟蹤到 *終售后的確保項(xiàng)目準(zhǔn)確到位,都有相關(guān)專業(yè)人士進(jìn)行維護(hù),確保您在合肥合肥星肽生物科技有限公司公司獲得*上等服務(wù)!
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聯(lián)系方式
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何經(jīng)理,合肥星肽生物科技有限公司,Tel:0551-63802898 400-8702-898,
E-mail:info@qingbio.com http://www.huangyuansheng.cn QQ:514713116
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1.) Pilakka-Kanthikeel S, Atluri VSR, Sagar V, Saxena SK, Nair M. (2013) "Targeted brain derived neurotropic factors (BDNF) delivery across the blood-brain barrier for neuro-protection using magnetic nano carriers: an in-vitro study." PLoS One. 8: e62241.
2.) Vijayaraghavan S, Karami A, Aeinehband S, Behbahani H, Grandien A, Nilsson B, Ekdahl KN, Lindblom RP, Piehl F, Darreh-Shori T. (2013) "Regulated Extracellular Choline Acetyltransferase Activity - The Plausible Missing Link of the Distant Action of Acetylcholine in the Cholinergic Anti-Inflammatory Pathway." PLoS One. 8: e65936
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5.) Triscott J, Lee C, Hu K, Fotovati A, Berns R, Pambid M, Luk M, Kast RE, Kong E, Toyota E, Yip S, Toyota B, Dunn SE. (2012) "Disulfiram, a drug widely used to control alcoholism, suppresses the self-renewal of glioblastoma and over-rides resistance to temozolomide." Oncotarget. 3: 1112-23.
6.) Robinson JP, Vanbrocklin MW, Lastwika KJ, McKinney AJ, Brandner S, Holmen SL. (2010) 'Activated MEK cooperates with Ink4a/Arf loss or Akt activation to induce gliomas in vivo.'
7.) Robinson JP, Vanbrocklin MW, Lastwika KJ, McKinney AJ, Brandner S, Holmen SL. (2010) "Activated MEK cooperates with Ink4a/Arf loss or Akt activation to induce gliomas in vivo." Oncogene. 30: 1341-50.
1、
Question:
Is it possible to freeze aliquots of complete medium to avoid that growth factors will become unstable at 4°C if the medium stays open longer than 1 month?thanks
Answers:
From our FAQ section: We do not recommend subjecting growth supplements to freeze-thaw, as this can result in reduced activity of components and the potential for components to irreversibly precipitate. Additionally, We do not recommend freezing media, as this can result in irreversible precipitation of media components.
2、Question:
Hi, is it recommended to grow HA in 2% FBS (10ml of FBS provided in the kit)? I am working with Astrocytes for the first time and wanted to make sure that I am doing everything right. So basically to make the complete medium, I add 2% FBS, 1% P/S and 1% growth supplement? Thanks!
Answers:
?Thanks for asking! Your process is correct.