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Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells

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產品名稱: Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells
產品型號: T0039
產品展商: ABM
產品價格: 0.00 元
產品文檔: 無相關文檔

簡單介紹

Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells T0039 永生化小鼠Floxed Fam20c牙乳頭間充質細胞 上海 安徽 合肥 ABM細胞庫


Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells  的詳細介紹

Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells T0039

永生化小鼠Floxed Fam20c牙乳頭間充質細胞 上海 安徽 合肥 ABM細胞庫

15000元  貨期2周


Print Version
BioSafety Level II
Organism Postnatal 4-day Fam20cf/fMice
Source Organ First molars
Growth Properties Adherent
Morphology Polygonal
Recommended Seeding Density Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications For Research Use Only
Immortalization Method Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
Description Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells are a reliable tool to study odontoblasts and the roles in formation, mineralization and repair of dentin. The cells are a precursor and can be differentiated into odonoblasts for further study. They carry the floxed Fam20c allele which allows further manipulation on exon 6-9 by Cre recombinase to obtain null Fam20c allele cells. Fam20c expresses the FAM20C kinase which has been associated with bone-related diseases due to mineralization dysfunction. The Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells can be expanded in vitro for research on the field of mineralization disorders.
Procedure Overview
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Propagation Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow VIII medium available from ABM (TM018). To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
Quality Control Immunohistochemistry for gene expression associated with the differentiation and mineralization of odontoblasts
Disclaimer 1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
關鍵字 Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells ;Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells ;Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells ;Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells ;Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells ;Immortalized Mouse Floxed Fam20c Dental Papillar Mesenchymal Cells 


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