Immortalized Human Microglia - SV40
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產品名稱: Immortalized Human Microglia - SV40
產品型號: T0251
產品展商: ABM
產品文檔: 無相關文檔
簡單介紹
Immortalized Human Microglia - SV40
永生化人小膠質細胞 - SV40 上海 安徽 合肥 ABM細胞庫
Immortalized Human Microglia - SV40
的詳細介紹
Immortalized Human Microglia - SV40
永生化人小膠質細胞 - SV40 上海 安徽 合肥 ABM細胞庫
15000元 貨期2周
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Print Version
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BioSafety Level
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II
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Organism
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Homo sapiens
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Source Organ
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Brain
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Growth Properties
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Adherent
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Morphology
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Polygonal
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Recommended Seeding Density
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Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
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Applications
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For Research Use Only
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Immortalization Method
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Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
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Cell Type Characterization
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Description
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Microglia cells are resident macrophages of the brain and spinal cord and they act as the first and the main form of active immune defense in the nervous system. The Immortalized Human Microglia - SV40 cell line is derived from Primary Human Microglia Cells (>99% purity) and have maintained microglial specific markers such as NGF, CD68 and TREM2 as demonstrated by RT-PCR. These cells are suitable for studies of human microglia in health and disease.
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Procedure Overview
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Image
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Propagation
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Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available in ABM, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% and 1% Penicillin/Streptomycin (G255). This cell line needs to be cultured in collagen coated flasks. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. Temperature: 37.0°C.
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Quality Control
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-
Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.
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Disclaimer
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1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
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關鍵字
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公司簡介
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合肥星肽生物科技有限公司作為專業的ABM中國代理商,專業經營原裝進口胎牛血清、細胞因子、ELISA試劑盒、細胞、抗體、生物試劑、耗材、培養基、一抗、二抗等。ABM安徽代理的產品涉及分子生物學、細胞生物學、細菌學、遺傳學、免疫學、生物化學、蛋白質學、細胞治療、臨床應用等領域。合肥星肽生物科技有限公司始終致力于為客戶提供可靠的研究材料以及方便快捷的服務,對購買項目的前期資料提供,中期合同保證,后期貨物跟蹤到*終售后的確保項目準確到位,都有相關專業人士進行維護,確保您在合肥合肥星肽生物科技有限公司公司獲得*上等服務!
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聯系方式
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何經理,合肥星肽生物科技有限公司,Tel:0551-63802898 400-8702-898,
E-mail:info@qingbio.com http://www.huangyuansheng.cn QQ:514713116
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關于合肥星肽生物科技有限公司
合肥星肽生物科技有限公司是一家致力于生命科學和生物技術領域的高科技企業,公司地址位于科教名城———合肥市。公司主營多肽合成、多肽定制、多肽純化等多肽制備多肽技術服務。在合肥市與中科院的關懷下,2021年初合肥市高新區與中科院分別與合肥星肽生物科技有限公司簽訂了戰略投資協議。
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