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Print Version
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BioSafety Level
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II
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Organism
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Rattus norvegicus
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SourceOrgan
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Aorta, Thoracic Smooth Muscle
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Growth Properties
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Adherent
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Morphology
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Fibroblast
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Description
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SSAO (semicarbazide-sensitive amine oxidase) has been associated with diabetes, Alzheimer’s, atherosclerosis, and stroke. Thus, it is necessary to have a reliable source of cells which expresses the desired protein for studies in its role and activity in pathways. The hSSAO/VAP-1 A7r5 Stable Cell Line stably expresses SSAO/VAP-1 in the plasma membrane, and releases SSAO into the culture medium. This cell line does not exhibit MAO-A nor MAO-B activities. It is a valuable source for studies in SSAO/VAP-1 membrane-bound form enzyme as a therapeutic target.
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Procedure Overview
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Recommended Seeding Density
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Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
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Applications
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For Research Use Only
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Over Expression
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Human SSAO/VAP-1
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Propagation
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Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium to a final concentration of 10% fetal bovine serum (TM999), 2 mM L-glutamine (G275) and Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
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Pictures
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Pictures
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Pictures
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Quality Control
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1) Selection via antibiotic G418; 2) Western blot used to determine presence of SSAO; 3) Activity of SSAO, MAO-A, and MAO-B was detected by radiochemical methods
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References
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Solé, M., Hernandez, M., Boada, M., & Unzeta, M. (2007). Characterization of A7r5 cell line transfected in a stable form by hSSAO/VAP-1 gene (A7r5 hSSAO/VAP-1 cell line). Journal of Neural Transmission J Neural Transm, 114(6), 763-767. doi:10.1007/s00702-007-0685-9
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Disclaimer
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1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers. All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
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關(guān)鍵字
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hSSAO/VAP-1 A7r5 Stable Cell Line;hSSAO/VAP-1 A7r5 Stable Cell Line;hSSAO/VAP-1 A7r5 Stable Cell LinehSSAO/VAP-1 A7r5 Stable Cell LinehSSAO/VAP-1 A7r5 Stable Cell Line;hSSAO/VAP-1 A7r5 Stable Cell Line;hSSAO/VAP-1 A7r5 Stable Cell Line
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公司簡介
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合肥星肽生物科技有限公司作為專業(yè)的ABM中國代理商，專業(yè)經(jīng)營原裝進(jìn)口胎牛血清、細(xì)胞因子、ELISA試劑盒、細(xì)胞、抗體、生物試劑、耗材、培養(yǎng)基、一抗、二抗等。ABM安徽代理的產(chǎn)品涉及分子生物學(xué)、細(xì)胞生物學(xué)、細(xì)菌學(xué)、遺傳學(xué)、免疫學(xué)、生物化學(xué)、蛋白質(zhì)學(xué)、細(xì)胞治療、臨床應(yīng)用等領(lǐng)域。合肥星肽生物科技有限公司始終致力于為客戶提供可靠的研究材料以及方便快捷的服務(wù)，對購買項目的前期資料提供,中期合同保證,后期貨物跟蹤到*終售后的確保項目準(zhǔn)確到位，都有相關(guān)專業(yè)人士進(jìn)行維護(hù),確保您在合肥合肥星肽生物科技有限公司公司獲得*上等服務(wù)！
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聯(lián)系方式
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何經(jīng)理，合肥星肽生物科技有限公司，Tel：0551-63802898 400-8702-898，
E-mail：info@qingbio.com http://www.huangyuansheng.cn QQ:514713116
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