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Print Version
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Safety
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BioSafety Level II
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Organism
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Human
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Source Organ
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Fetal Lung Fibroblasts (MRC-5)
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Picture
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Image
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Concentration
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106Cells/ml
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Induction
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iPS-14 was generated using lentiviral transduction of human fetal lung fibroblasts with cMYC, KLF4, OCT4 and SOX2.
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Pluripotency
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Positive for markers Nanog, Tra-1-60, Tra-1-80 and SSEA-3 by immunofluorescence and flow cytometry. Capable of differentiating into derivatives of the 3 germ layers.
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Propagation
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The base medium for this cell line is Prigrow IV medium available from ABM (TM004). To make the complete growth medium, add the following components to the base medium: serum replacer to a final concentration of 20%; b-FGF to a final concentration of 6ng/ml; 1 mM L-glutamine and 2-mercaptoethanol solution; 1X Non-essential amino acids. Sterilize the media through a 0.22 micron filter before use. This cell line needs to be cultured on mouse embryonic fibroblasts (ABM T2001) feeder cells. Atmosphere:air: 95%, CO2: 5%; Temperature: 37.0°C.
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Subculturing
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1. Remove and discard culture medium.
2. Add 2.0mL of Dispase solution to flask and incubate the cells at 37°C to facilitate dispersal. Observe cells under an inverted microscope until cell layer is dispersed.
3.Transfer the dispase solutions containing colonies into a conical tube.
4. Centrifuge cells at 1000rpm for 3 minutes to pellet.
5. Gently aspirate out the solution, leaving pellet undisturbed.
6. Resuspend pellet in fresh culture medium and plate on MEF feeder plates.
7. Incubate cultures at 37°C.
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Storage
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-180°C
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Shipping
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On Dry Ice
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Notes
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For Research Use Only, not for therapeutic or diagnostic purposes.
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References
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1. Stuart M Chambers, Christopher A Fasano, Eirni P Papapetrou, Mark Tomishima, Michel Sadelain and Lorenz Studer. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol. 26, 275-280 (2009).
2. Papapetrou EP, Tomishima MJ, Chambers SM, Mica Y, Reed E, Menon J, Tabar V, Mo Q, Studer L, Sadelain M. Stoichiometric and temporal requirements of Oct4, Sox2, Klf4, and c-Myc expression for efficient human iPSC induction and differentiation. Proc Natl Acad Sci U S A. Aug 4;106(31):12759-64. (2009).
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關鍵字
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iPS-14 Cells;iPS-14 Cells;iPS-14 Cells;iPS-14 Cells;iPS-14 Cells;iPS-14 Cells
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公司簡介
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合肥星肽生物科技有限公司作為專業的ABM中國代理商,專業經營原裝進口胎牛血清、細胞因子、ELISA試劑盒、細胞、抗體、生物試劑、耗材、培養基、一抗、二抗等。ABM安徽代理的產品涉及分子生物學、細胞生物學、細菌學、遺傳學、免疫學、生物化學、蛋白質學、細胞治療、臨床應用等領域。合肥星肽生物科技有限公司始終致力于為客戶提供可靠的研究材料以及方便快捷的服務,對購買項目的前期資料提供,中期合同保證,后期貨物跟蹤到*終售后的確保項目準確到位,都有相關專業人士進行維護,確保您在合肥合肥星肽生物科技有限公司公司獲得*上等服務!
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聯系方式
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何經理,合肥星肽生物科技有限公司,Tel:0551-63802898 400-8702-898,
E-mail:info@qingbio.com http://www.huangyuansheng.cn QQ:514713116
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